FIGURE

Fig. 9

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ZDB-FIG-080425-9

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Lieschke et al., 2002 - Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning

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Fig. 9

Expression of spi1, fli1, and scl in wild-type and chordino (din) zebrafish embryos. (A, B) Flat-mount 12-somite wild-type (A) and chordino (B) embryos stained with spi1 riboprobe. Embryos were opened in the middle of their backs, deyolked, and flat mounted to display ventral views of the discontinuous rostral (top) and caudal (bottom) ends of the embryo. In chordino, the number of rostral spi1⁺ cells is not reduced, and they are more dispersed. (C–F) Wild-type (C, E) and chordino (D, F) embryos stained with scl (blue in C, D) or fli1 (blue in E, F) and spi1 (red in C–F) riboprobes. Although the rostral LPM is somewhat reduced in size in chordino, it still makes cell types expressing these three markers, while the caudal LPM is greatly enlarged. The extent of caudal of spi1-expression was not reduced in chordino mutants, although it was altered in shape; this domain of expression is more completely shown in (D) and (F), rather than in (B). Scale bars, 100 μm.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 246(2), Lieschke, G.J., Oates, A.C., Paw, B.H., Thompson, M.A., Hall, N.E., Ward, A.C., Ho, R.K., Zon, L.I., and Layton, J.E., Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning, 274-295, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.