FIGURE

Fig. 3

ID

ZDB-FIG-080425-3

Publication

Lieschke et al., 2002 - Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning

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Fig. 3

Expression of spi1 relative to gata1 in the lateral plate mesoderm. Two-color in situ hybridization gene expression analysis of wild-type zebrafish embryo (12 somite, flat mount, rostral up). (A)Alow-power view spanning the entire lateral plate mesoderm (LPM). (B, C) High-magnification views (63x water immersion lens) of regions boxed in (A), in which the expression of spi1 has been detected by fluorescence of Fast Red using a RITC filter set. (B) spi1⁺gata1^- cells in the rostral LPM. Arrowheads indicate the caudal margin of the eye. (C) spi1⁺gata1⁺ cells in the caudal LPM. Arrowhead indicates an indentation to the otherwise uniform LPMgata1⁺ domain, due to several cells that express neither gata1 nor spi1. Arrow indicates a lateral irregularity in the LPM gata1 domain, due to several cells that express both gata1 and spi1. Scale bar in (B), 30 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 246(2), Lieschke, G.J., Oates, A.C., Paw, B.H., Thompson, M.A., Hall, N.E., Ward, A.C., Ho, R.K., Zon, L.I., and Layton, J.E., Zebrafish SPI-1 (PU.1) marks a site of myeloid development independent of primitive erythropoiesis: implications for axial patterning, 274-295, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.