Fig. 2
- ID
- ZDB-FIG-080424-64
- Publication
- Hall et al., 2002 - Radar is required for the establishment of vascular integrity in the zebrafish
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Forced radar expression elicits ventralized phenotypes. (A) Lateral view of a 24-hpf embryo following the injection of pCS2/EGFP (75-pg dose). The extent of mosaic EGFP expression is depicted as a superimposed green pattern in (A–C). (B) Lateral view of a mildly ventralized 24-hpf embryo (V1–V2) following the coinjection of P2RAD and pCS2/EGFP (75-pg dose). Low-level GFP expression is present in the posterior ICM region. Black arrowhead and arrow denote reduction of head structures and expanded ICM compartment, respectively. (C) Lateral view of a severely ventralized 24-hpf embryo (V4) following the coinjection of P2RAD and pCS2/EGFP (75-pg dose). Widespread GFP expression can be seen in the trunk. Small black arrowhead and arrow denote absent anterior head structures and expanded ICM region, respectively. Large black arrowhead denotes fused somites. (D) Whole-mount in situ hybridization staining for flk-1 in a 24-hpf wild type embryo and its cross-section through the developing tail (E). Arrow and arrowhead in (E) denote primitive caudal artery and caudal vein, respectively. (F) Expression of flk-1 in a mildly ventralized 24-hpf P2RAD/EGFP coinjected embryo (75-pg dose) and its transverse section through the tail (G). Arrows in (G) denote expanded flk-1 expression domain. N, notochord. Scale bars in (A, C) and (E, G) represent 200 and 100 μm, respectively. |
Reprinted from Developmental Biology, 251(1), Hall, C.J., Flores, M.V.C., Davidson, A.J., Crosier, K.E., and Crosier, P.S., Radar is required for the establishment of vascular integrity in the zebrafish, 105-117, Copyright (2002) with permission from Elsevier. Full text @ Dev. Biol.