FIGURE

Fig. 2

ID

ZDB-FIG-080423-6

Publication

Malicki et al., 2003 - Zebrafish N-cadherin, encoded by the glass onion locus, plays an essential role in retinal patterning

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Fig. 2

Knockdown phenotype of N-cadherin. Anti-N-cadherin morpholino-modified antisense oligonucleotides reproduce the glass onion phenotype. Gross morphology of the wild type (A), glo^m117 (B), and anti-N-cadherin morpholino-treated animals (C) at 36 hpf. Eye pigmentation of the wild type (D), glo^m117 (E), and morpholino-treated animals (F) at 48 hpf. Tail and somites of the wild type (G), glo^m117 (H), and morpholino-treated animals (I) at 30 hpf. Somite boundaries are poorly defined in both mutant and morpholino-treated embryos. Analysis of cryosections indicates that retinal neuroepithelium is severely disorganized at 28 hpf in morpholino-treated animals (K, L compare with the wild type in J). In (J–L), centrosomes are stained with anti-γ-tubulin antibody (blue), adherens-junction associated actin foci are visualized with Alexa-488-conjugated phalloidin (green), while M- phase nuclei are identified by anti-phosphohistone antibody staining (red). Embryos in (K) and (L) were treated with anti N-cadherin morpholinos 2 and 3, respectively. In (G-I) anterior is right and dorsal is top. “le” in (J–L) indicates lens. Arrows indicate the midline.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 259(1), Malicki, J., Jo, H., and Pujic, Z., Zebrafish N-cadherin, encoded by the glass onion locus, plays an essential role in retinal patterning, 95-108, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.