Fig. 6
- ID
- ZDB-FIG-080411-14
- Publication
- Chen et al., 2008 - Liver-specific expression of p53-negative regulator mdm2 leads to growth retardation and fragile liver in zebrafish
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Immunohistochemical staining of active caspase 3 and proliferating cell nuclear antigen (PCNA) on the liver sections of 4-month Gmdm2-liver and G-liver transgenic zebrafish.A-C: Detection of apoptotic cells in normal liver of 4-month G-liver (A) and hypoplastic liver of 4-month Gmdm2-liver #1 (B) and Gmdm2-liver #2 (B) by immunohistochemical staining of active caspase 3 and haematoxylin counterstain. The active caspase 3 immunopositive (brown reaction product) cells were considered to be apoptotic cells. Comparison of the intensity of nuclear staining and the number of active caspase 3 immunopositive cells in the normal (A) and hypoplastic (B and C) livers revealed a significantly increased level of cell death in the hypoplastic livers of 4-month Gmdm2-liver #1 and Gmdm2-liver #2 where GFP::Mdm2 is over-expressed. D-F: Detection of the proliferative activity of cells in normal liver of 4-month G-liver (D) and hypoplastic liver of 4-month Gmdm2-liver #1 (E) and Gmdm2-liver #2 (F) by immunohistochemical staining of proliferating cell nuclear antigen (PCNA) and haematoxylin counterstain. The PCNA immunopositive (brown reactth G-liver (D) and hypoplastic liver of 4-month Gmdm2-liver #1 (E) and Gmdm2-liver #2 (F) by immunohistochemical staining of proliferion product) cells were considered to be proliferative cells. Comparison of the normal (D) and hyperplastic (E and F) livers revealed a cell-cycle arrest or decreased number of immunopositive cells in the hypoplastic livers of 4-month Gmdm2-liver #1 and Gmdm2-liver #2 where GFP::Mdm2 is over-expressed. Arrows indicate examples of immunopositive (brown reaction product) cells. Scale bar = 50 μm. |