FIGURE

Fig. S5

ID

ZDB-FIG-080402-12

Publication

Blechman et al., 2007 - Specification of hypothalamic neurons by dual regulation of the homeodomain protein Orthopedia

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Effects of otpa antisense RNA probes and otpb mRNA. Wild-type (WT; a) embryos and their too few^m808 (tof^m808) mutant siblings (B) were fixed at 52 hours post fertilization and subjected to whole-mount in situ hybridization with antisense RNA probes directed against otpa followed by immunostaining with an antibody directed against tyrosine hydroxylase (TH). Black arrow heads mark deficient otpa expression domains. (C-F) Embryos were injected with either a buffer solution (Control, C,E) or otpb mRNA (E,F) and were thereafter (48 hours post injection) subjected to either in situ hybridization with probes directed to either pac1 (C,D) or pacap1b (E,F). HB, hindbrain; PT, posterior tuberculum; Tel, telencephalon. Scale bars, 50 μm.

Expression Data

Genes:	adcyap1b adcyap1r1b otpa th
Fish:	WT fezf2^m808/m808
Anatomical Terms:	caudal tuberculum hindbrain hypothalamus preoptic area telencephalon
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development