Whole-mount in situ hybridization for mmrn2b in zebrafish embryos at different developmental stages. A: Expression in the posterior region of the tail (arrow) at 15 somites (s). B: Expression at 22 hours postfertilization (hpf) in ependymal cells lining the midbrain ventricle. C: Expression in the heart at 22 hpf. D: Expression in the inner ear at 22 hpf. E: Expression in posterior somites at 22 hpf. F: Expression in the mesenchyme of fin buds at 72 hpf. Except for embryo in A, yolk was removed and embryos were mounted between glass coverslips. In A, anterior side of embryo is at the top; in B-F, anterior side is on the left. A and E are lateral views; B-D and F are dorsal view. fb, fin bud; h, heart; ie, inner ear; mb, midbrain; opt, optic cup; so, somites; yo, yolk.
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