FIGURE

Fig. 6

ID

ZDB-FIG-080326-47

Publication

Isken et al., 2008 - RBP4 Disrupts Vitamin A Uptake Homeostasis in a STRA6-Deficient Animal Model for Matthew-Wood Syndrome

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Fig. 6

PTU and rbp4-MO Treatments Prevent Developmental Abnormalities in stra6 Morphants
(A) rbp4 mRNA expression (blue) was reduced in PTU-treated embryos.
(B) Retinoids (retinaldehyde and RE) of the head and trunk of 4 dpf control and PTU- and rbp4-MO-treated larvae. Values represent the means ± SD of three independent experiments. ^*p < 0.02 versus control by Student′s t test.
(C) Immunoblot analysis of Rbp4 levels in 24 hpf embryos treated with PTU, PTU and MO2 (left), and rbp4-MO (right) as compared to respective controls. Ponceau S red-stained membranes are shown as a loading control (lower panel).
(D) stra6 morphants and controls were raised in the presence and absence of PTU (n = 170 each). Representative photographs of 3 dpf larvae are shown. PTU treatment prevented developmental abnormalities in 73% of the stra6 morphants. In the absence of PTU, all stra6 morphants displayed cardiac edema (asterisk).
(E) Larvae raised from oocytes either injected with rbp4-MO or coinjected with rbp4-MO and MO2. These morphant larvae were raised in the absence of PTU. rbp4 morphant larvae showed no developmental abnormalities (n = 120). Coinjection of rbp4-MO and MO2 largely prevented developmental abnormalities in 77% of the compound morphants (n = 120).
Scale bar = 100 μM in (A) and 500 μm in (D) and (E). Anterior is to the left in all pictures.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL AB/TL + MO2-stra6 AB/TL + MO3-rbp4 AB/TL + MO2-stra6 + MO3-rbp4
Condition:	chemical treatment: N-phenylthiourea
Knockdown Reagents:	MO2-stra6 MO3-rbp4
Observed In:	embryo development heart pericardium
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Cell Metabolism, 7(3), Isken, A., Golczak, M., Oberhauser, V., Hunzelmann, S., Driever, W., Imanishi, Y., Palczewski, K., and von Lintig, J., RBP4 Disrupts Vitamin A Uptake Homeostasis in a STRA6-Deficient Animal Model for Matthew-Wood Syndrome, 258-268, Copyright (2008) with permission from Elsevier. Full text @ Cell Metab.