FIGURE

Fig. 9

ID

ZDB-FIG-080325-42

Publication

Monteiro et al., 2008 - Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish

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Fig. 9

Bmpr2a and bmpr2b show different binding and Smad-dependent signalling transduction properties in vitro. (A) Co-immunoprecipitation of radioactive BMP2 ([¹²⁵I]-BMP2) with overexpressed bmpr2a-myc, bmpr2b-Δic: :GFP and ALK3-HA in COS-7 cells. In the absence of ALK3, no binding of bmpr2a or bmpr2b to [¹²⁵I]-BMP2 was detected. Bmpr2a but not bmpr2b co-immunoprecipitates with [¹²⁵I]-BMP2 in the presence of ALK3-HA. (B) Direct western blot on cell extracts used for co-immunoprecipitation with antibodies against myc (bmpr2a), GFP (bmpr2b-Δic) and HA (ALK3) shows correct expression of the bmpr2a-myc, bmpr2b-Δic: :GFP and ALK3-HA constructs. (C) Transient transfection of bmpr2a, bmpr2b and bmpr2a + bmpr2b with or without BMP4 (50 ng/ml) in osteoblast progenitor cells. RLU, Relative Luciferase Units. These experiments have been repeated at least twice.

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 315(1), Monteiro, R., van Dinther, M., Bakkers, J., Wilkinson, R., Patient, R., Ten Dijke, P., and Mummery, C., Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish, 55-71, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.