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Fig. 3

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ZDB-FIG-080325-33

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Monteiro et al., 2008 - Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish

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Fig. 3

Injection of truncated bmpr2a and bmpr2b mRNA induce BMP loss-of-function phenotypes. Morphological phenotypes were classified according to Kishimoto and colleagues (Kishimoto et al., 1997). (A) Uninjected embryo at tailbud stage. Embryos were injected with 100 ng/μl of bmpr2a-Δic (B, E, H) or 100 ng/μl of bmpr2b-Δic (C, F, I). Injection of bmpr2a-Δic (B) or bmpr2b-Δic (C) induced an enlarged notochord at tailbud stage (red arrows). (D) Wild type embryo at 24 hpf. (E) Example of C2–C4 dorsalized phenotypes after injection of bmpr2a-Δic mRNA and (F) C2–C3 phenotypes after injection of bmpr2b-Δic mRNA. (G) Wild type tail at 24 hpf. (H) Partial tail duplication in bmpr2a-Δic-injected embryo. (I) Cloacal cysts appear frequently upon bmpr2a-Δic or bmpr2b-Δic injection. Anterior is to the left (A–I). Quantification of the number of embryos showing dorsalized phenotypes in each category (C1–C5) upon injection of 50 and 100 ng/μl bmpr2a-Δic (J) or bmpr2b-Δic mRNA (K). n - number of injected embryos at each concentration.

Expression Data

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Antibody Labeling

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Acknowledgments

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Reprinted from Developmental Biology, 315(1), Monteiro, R., van Dinther, M., Bakkers, J., Wilkinson, R., Patient, R., Ten Dijke, P., and Mummery, C., Two novel type II receptors mediate BMP signalling and are required to establish left-right asymmetry in zebrafish, 55-71, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.