ZFIN ID: ZDB-FIG-071120-8
Webb et al., 2007 - Laminin alpha5 is essential for the formation of the zebrafish fins. Developmental Biology   311(2):369-382 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-25 to Days 7-13

Fig. 1 m538 mutants have severe fin and yolk extension defects. (A–D) Reductions in median fins are shown at 36 hpf (A, C), 48 hpf (B) and 7 dpf (D). In panel D, the dotted line shows the outer limit of the wild-type fin fold. Mutants often contain reduced or absent yolk extensions (B, arrows). (E–H) Anterior tissues develop normally in the mutants, with the exception of the pectoral fins. Defects in pectoral fin outgrowth can be detected at 48 hpf by immunostaining with a pan-cadherin antibody (red, E, F; green are nuclei stained with DAPI and pseudo-colored green for contrast). At 7 dpf, pectoral fins are short and stunted in m538 mutants (arrowheads in panels G and H). (A–F) Lateral views and (G, H) dorsal views; anterior view is to the left in all images.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lama5m538/m538(AB) standard conditions Prim-25 extension hypoplastic, abnormal
Prim-25 medial fin development disrupted, abnormal
Prim-25 median fin fold structure, abnormal
Long-pec extension aplastic, abnormal
Long-pec medial fin development disrupted, abnormal
Long-pec median fin fold structure, abnormal
Long-pec pectoral fin development disrupted, abnormal
Long-pec pectoral fin structure, abnormal
Days 7-13 medial fin development disrupted, abnormal
Days 7-13 median fin fold structure, abnormal
Days 7-13 pectoral fin decreased size, abnormal
Days 7-13 pectoral fin development disrupted, abnormal
Days 7-13 pectoral fin structure, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 311(2), Webb, A.E., Sanderford, J., Frank, D., Talbot, W.S., Driever, W., and Kimelman, D., Laminin alpha5 is essential for the formation of the zebrafish fins, 369-382, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.