ZFIN ID: ZDB-FIG-071120-11
Webb et al., 2007 - Laminin alpha5 is essential for the formation of the zebrafish fins. Developmental Biology   311(2):369-382 Full text @ Dev. Biol.
Observed In:
Stage: Long-pec

Fig. 5 Laminin α5 is required for localization of actin and β-catenin to cell–cell boundaries. Confocal line scans (transverse sections) of the fin fold at 48 hpf in wild-type (A–D) and lama5 mutant (E–H) embryos. Nuclei are marked with DAPI (A, E) and fin folds are bracketed. Neural tube and somitic nuclei are to the right of the bracket in panel E. In wild-type embryos, β-catenin (green, B) and actin (red, C) are enriched at cell–cell boundaries (arrowheads and inset in panel D). Lama5 mutants have reduced fin fold extension (compare brackets in panels A and E) and fail to enrich β-catenin (F) and actin (G) at points of cell–cell contact (inset in H). Merged green and red channels are shown in panels D and H, dorsal is to the left.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lama5m538/m538(AB) standard conditions Long-pec median fin fold adherens junction composition, abnormal
Long-pec median fin fold epidermal cell positional polarity, abnormal
Long-pec protein localization to adherens junction arrested, abnormal
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 311(2), Webb, A.E., Sanderford, J., Frank, D., Talbot, W.S., Driever, W., and Kimelman, D., Laminin alpha5 is essential for the formation of the zebrafish fins, 369-382, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.