FIGURE

Fig. 3

ID

ZDB-FIG-071115-6

Publication

Villefranc et al., 2007 - Gateway compatible vectors for analysis of gene function in the zebrafish

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Fig. 3

Validation of Gateway compatible N-term pCS vectors. A,B: Embryo injected with myc-notch3icd mRNA from plasmid generated through standard ligation-mediated cloning. C,D: Embryos injected with myc-notch3icd mRNA from plasmid generated through Gateway cloning (GW). A,C: Embryos fixed at 50% epiboly and immunostained to detect the myc epitope; dorsal view. B,D: Live embryos at 26 hpf, lateral view, anterior to the left. E-G: Embryos injected with mRNA encoding mCherry-Notch3ICD. H-J: Embryos injected with mRNA encoding Egfp-Notch3ICD. K-N: Embryos injected with mRNA encoding an mCherry-Egfp fusion. E,H,K: Dorsal views at 50% epiboly, images generated under standard transmitted light illumination of live embryos. F,L: Visualization of red fluorescence at 50% epiboly, dorsal views. I,M: Visualization of green fluorescence, dorsal views at 50% epiboly. G,J,N: Lateral views under transmitted light, dorsal is up, anterior to the left. All embryos injected with 100 pg of indicated mRNA. N3ICD, notch3 intracellular domain.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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