FIGURE

Fig. 4

ID

ZDB-FIG-071001-27

Publication

Lee et al., 2006 - Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud

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Fig. 4

Splice junction targeted anti-blimp-1 MOs effectively block splicing of blimp-1 pre-mRNA. (A) RT-PCR of mRNA extracted from wild-type embryos and blimp-1 morphants, showing the expected 783 bp band in the wild-type lanes and an approximately 3 kb band in those of the morphants. The primer pair used for the PCR reaction amplifies across exons 1-5 (see Materials and methods). (B) Diagram illustrating the target site of the MO, at the junction between exon 2 and intron 2. The last codon of exon 2 and the first codon of exon 3, together with their corresponding amino acid, are indicated. The premature stop codon in the mis-spliced blimp-1 mRNA is highlighted in red.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 300(2), Lee, B.C., and Roy, S., Blimp-1 is an essential component of the genetic program controlling development of the pectoral limb bud, 623-634, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.