Fig. 9
Delayed Somite Segmentation Defects following a Heat-Shock?Induced Pulse of HA-Her7. The photographs show effects of a 60-min heat shock at 37 °C initiated at the five-somite stage, followed by 7 h of recovery at the normal incubation temperature, for (A) an hsp70:HA-her7 transgenic and (B) a wild-type sibling control. In the transgenic, four somites (s6 to s9) have formed normally after the onset of heat shock, but a block of somites subsequent to that has been disrupted (arrowhead). Embryos are lightly fixed, but unstained, and viewed with dark-field illumination. |