FIGURE

Fig. 4

ID

ZDB-FIG-070118-7

Publication

Lin et al., 2006 - Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish

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Fig. 4

Myod is required for the development of posterior extraocular recti and ventral branchial muscles. Embryos derived from the transgenic line Tg(α-actin:RFP) (A–D) and from the wild-type strain (E–J) were used. Embryos injected with 4 ng of myod-morpholino oligonucleotide (MO) to inhibit specifically myod translation were followed to observe the development of cranial muscle (B, D) and the expression of myf5 (F, H) and myogenin (J; lateral views: A, B, E–J; ventral views: C, D) at the stages indicated. The anterior extraocular recti (io and so), dorsal branchial muscle (ah, ao, do, and lap), and sh developed normally in the myod transgenic morphant (A vs. B; C vs. D). The posterior extraocular (sr, mr, ir, and lr) and the ventral branchial muscle (ima, imp, ih, and hh) were totally lost. When wild-type embryos were injected with myod-MO, myf5 was expressed normally in the myod morphants at 30 hpf (E vs. F) and at 36 hpf (G vs. H). Whereas, the expression of myogenin was decreased in myod morphants at 58 hpf (I vs. J), indicating a reduction in myogenin-positive muscle fibers.

Expression Data

Genes:	myf5 myog
Fish:	WT WT + MO1-myod1
Knockdown Reagent:	MO1-myod1
Anatomical Terms:	cephalic musculature dorsal oblique extraocular muscle dorsal rectus lateral rectus pharyngeal arch ventral oblique extraocular muscle
Stage Range:	Prim-15 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-myod1
Knockdown Reagent:	MO1-myod1
Observed In:	cephalic musculature
Stage Range:	Prim-15 to Long-pec

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 299(2), Lin, C.Y., Yung, R.F., Lee, H.C., Chen, W.T., Chen, Y.H., and Tsai, H.J., Myogenic regulatory factors Myf5 and Myod function distinctly during craniofacial myogenesis of zebrafish, 594-608, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.