Fig. S6

Examples of other embryos showing mosaicism in the neuroepithelium. Two additional examples of mosaic embryos obtained by transplantations of WT>olt Isl1-GFP embryos. The donor cells were labeled with rhodamine-conjugated dextran (red). (A) In this mosaic embryo, the wild-type donor-cell cluster (red) was located in the lateral part of the neuroepithelium (asterisk) and the olt-derived nVII motor neurons (green) failed to invade this region (arrowheads in A). (B) In this mosaic embryo, the wild-type donor-cell cluster (red) was located in the medial part of the neuroepithelium (asterisk), and the olt-derived nVII motor neurons (green) were also excluded from this wild-type donor-cell cluster (arrowhead in B). The yellow signals in the merged panels are technical artifacts caused by superimposition of the red signals of the donor cells and the green signals of the nVII motor neurons. As red signals were not detected in the axons of the nVII motor neurons, all of the nVII motor neurons were derived from the olt host embryos. Images are composite stacks of serial optical sections, shown in dorsal view. See also Fig. 6H.