PUBLICATION

Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain

Authors
Wada, H., Tanaka, H., Nakayama, S., Iwasaki, M., and Okamoto, H.
ID
ZDB-PUB-061108-7
Date
2006
Source
Development (Cambridge, England)   133(23): 4749-4759 (Journal)
Registered Authors
Nakayama, Satomi, Okamoto, Hitoshi, Tanaka, Hideomi, Wada, Hironori
Keywords
Zebrafish, frizzled, celsr, Facial motor neuron, Neuroepithelium
MeSH Terms
  • Mutation
  • Neuroepithelial Cells/cytology
  • Neuroepithelial Cells/metabolism
  • Frizzled Receptors/genetics*
  • Frizzled Receptors/metabolism
  • Animals
  • Cell Movement/genetics
  • Cell Movement/physiology
  • Gene Expression Regulation, Developmental
  • Motor Neurons/cytology
  • Motor Neurons/metabolism
  • Rhombencephalon/embryology*
  • Rhombencephalon/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Animals, Genetically Modified
  • Cadherins/genetics*
  • Cadherins/metabolism
(all 21)
PubMed
17079269 Full text @ Development
Abstract
Migration of neurons from their birthplace to their final target area is a crucial step in brain development. Here, we show that expression of the off-limits/frizzled3a (olt/fz3a) and off-road/celsr2 (ord/celsr2) genes in neuroepithelial cells maintains the facial (nVII) motor neurons near the pial surface during their caudal migration in the zebrafish hindbrain. In the absence of olt/fz3a expression in the neuroepithelium, nVII motor neurons extended aberrant radial processes towards the ventricular surface and mismigrated radially to the dorsomedial part of the hindbrain. Our findings reveal a novel role for these genes, distinctive from their already known functions, in the regulation of the planar cell polarity (i.e. preventing integration of differentiated neurons into the neuroepithelial layer). This contrasts markedly with their reported role in reintegration of neuroepithelial daughter cells into the neuroepithelial layer after cell division.
Genes / Markers
Figures
Figure Gallery (13 images) / 2
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
celsr2rw71/rw71standard conditionsUnknown
celsr2rw71/rw71standard conditions14-19 somites to Prim-15
celsr2rw71/rw71standard conditionsPrim-5
celsr2rw71/rw71standard conditionsPrim-15
celsr2rw71/rw71standard conditionsPrim-15
celsr2rw71/rw71standard conditionsPrim-15
celsr2rw71/rw71standard conditionsLong-pec
celsr2rw71/rw71standard conditionsLong-pec
fzd3arw689/rw689standard conditionsUnknown
fzd3arw689/rw689standard conditions14-19 somites to Prim-15
1 - 10 of 16
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
celsr2_unspecified
    		Unspecified
    rw0TgTransgenic Insertion
      rw71
        		Point Mutation
        rw135
          		Point Mutation
          rw166
            		Point Mutation
            rw380
              		Point Mutation
              rw689
                		Point Mutation
                1 - 7 of 7
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                Human Disease / Model
                No data available
                Sequence Targeting Reagents
                Target Reagent Reagent Type
                celsr1aMO4-celsr1aMRPHLNO
                celsr1bMO3-celsr1bMRPHLNO
                celsr2MO1-celsr2MRPHLNO
                fzd3aMO1-fzd3aMRPHLNO
                1 - 4 of 4
                Show
                Fish
                Fish
                celsr2rw71/rw71
                celsr2rw135/rw135
                celsr2rw166/rw166
                celsr2rw380/rw380
                celsr2unspecified
                fzd3arw689/rw689
                rw0Tg
                rw0Tg + MO1-fzd3a
                WT
                1 - 9 of 9
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                Antibodies
                No data available
                Orthology
                Gene Orthology
                celsr2
                1 - 1 of 1
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                Engineered Foreign Genes
                Marker Marker Type Name
                GFPEFGGFP
                1 - 1 of 1
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                Mapping
                Entity Type Entity Symbol Location
                ESTwz12434Chr: 22 Details
                ESTzeh1122Chr: 22 Details
                Featurecelsr2_unspecifiedChr: 22 Details
                Featurerw689Chr: 20 Details
                GENEcelsr2Chr: 22 Details
                SSLPz8150Chr: 20 Details
                SSLPz9794Chr: 20 Details
                SSLPz11262Chr: 22 Details
                1 - 8 of 8
                Show
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                Citation
                Wada, H., Tanaka, H., Nakayama, S., Iwasaki, M., and Okamoto, H. (2006) Frizzled3a and Celsr2 function in the neuroepithelium to regulate migration of facial motor neurons in the developing zebrafish hindbrain. Development (Cambridge, England). 133(23):4749-4759.