FIGURE

Fig. 1

ID

ZDB-FIG-061115-4

Publication

Vallone et al., 2004 - E-box function in a period gene repressed by light

Other Figures

Fig. 1

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Fig. 1

RNase protection assay analysis of zfper4 (410-nt protected fragment, amino acid positions 88–225) and β-actin (282-nt protected fragment) was performed with RNA harvested at the indicated zeitgeber times (ZT0 is lights-on, ZT12 is lights-off) or CT. (A) Whole brain from adult zebrafish maintained in LD. (B) Six-day-old larvae raised in LD. (C) PAC-2 cells were maintained in LD for 5 days. Cells were entrained in LD and harvested in DD (D)orinLL(E). (F) Peak and trough samples from the LD, DD, and LL sets of PAC-2 cells (C–E) assayed together to allow comparison of the relative expression levels. (G) Larvae raised for 6 days in DD and then exposed to light for the indicated times (hours; + Light). Control larvae remained in DD and were harvested in parallel. (H) Analysis of PAC-2 cells equivalent to G.

Expression Data

Gene:	per1b
Fish:	WT
Conditions:	constant dark lighting conditions constant light
Anatomical Terms:	brain whole organism
Stage Range:	Day 6 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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