FIGURE

Fig. 8

ID

ZDB-FIG-060620-21

Publication

Cheung et al., 2006 - Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development

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Fig. 8

The use of aeq-mRNA extends the aequorin-based Ca2+ imaging window beyond 24 hpf. (A) A comparison of the aequorin-generated luminescence observed in representative aeqmRNA- and f-aequorin-injected embryos from 27-36 hpf. Traces were generated from data points representing 60 sec of luminescence that were acquired every 60 s for an imaging field covering approximately 64,500 pixels. (B) An example of a localized intercellular Ca2+ signal that is generated in the trunk at ~47 hpf. The panels show the representative pattern of luminescence superimposed on corresponding bright-field images. Each panel displays 60 s of accumulated light with a 30 s interval between each image. Ant. and Pos. represent anterior and posterior, respectively. Color scale indicates luminescent flux in photons/pixel. Scale bar, 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Dev. Biol.