FIGURE

Fig. 2

ID

ZDB-FIG-060620-17

Publication

Cheung et al., 2006 - Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development

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Fig. 2

Effect of aeq-mRNA and aeq::EGFP-mRNA on normal development. Embryos were injected with different amounts of aeq-mRNA or aeq::EGFP-mRNA at the 1-cell stage and then observed after 24 h. (A) Bar graph to illustrate the percentage of normal embryos that developed for each amount of mRNA injected. The data are expressed as mean ± SEM (n ≥3). (B) Bright-field images to show the effect of injecting different amounts of aeq-mRNA and aeq::EGFP-mRNA into embryos compared to (i) an uninjected control embryo at 24 hpf. Representative examples of embryos injected with (ii) 500 pg, (iii) 700 pg or (iv) 1500 pg aeq-mRNA, or with (v) 400 pg, (vi) 700 pg or (vii) 1500 pg aeq::EGFP-mRNA. Embryos injected with >700 pg aeq-mRNA or >400 pg aeq::EGFP-mRNA exhibited defects in the development of both head and tail. Scale bar, 200 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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