Fig. 3

 Temporal rescue of mini fin mutants by hsp-tld. (A–C) tld and (D–F) eve1 expression in transgenic mfn mutant embryos heat-shocked for 10 min during late gastrulation (85–90% epiboly). Expression of tld (N = 30) and eve1 (N = 15) in embryos fixed before the heat-shock treatment (A, D). Expression of tld 10 (N = 33) (B) or 30 (N = 43) (C) min after the start of the heat-shock. Expression of eve1 1 h after the start of the heat-shock in non-transgenic (E) and transgenic embryos (N = 15) (F). (G) Expression of myc-tagged Tld protein after a 10-min heat-shock, or in the last lane a 60-min heat-shock, during late gastrulation. Embryos were collected at stated time points following the start of the heat-shock period and assayed by anti-myc Western blot analysis. A lower non-specific cross-reacting band serves as a loading control. (H) Graphic representation of rescue of mfn transgenic embryos after a 10-min heat-shock treatment at different time points during development. The blue graph indicates an average of the percent rescue seen in multiple transgenic lines heat-shocked. The red graph shows a “sub-optimal” 7-min heat-shock performed in transgenic line 75, so that less than 100% of the transgenic embryos at any time point were rescued.