FIGURE

Fig. 1

ID

ZDB-FIG-060112-1

Publication

Lee et al., 2006 - Vascular endothelial growth factor receptor signaling is required for cardiac valve formation in zebrafish

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Fig. 1

VEGF-induced NFATc1 nuclear translocation was blocked by the VEGF-R tyrosine kinase inhibitors. HPVEC grown for 24 hr in Endothelial Basal Media media with 10% FBS were either mock stimulated (a) or stimulated with 50 ng/ml VEGF₁₆₅ (R&D Systems) for 30 min (b-f). Intracellular localization of NFATc1 was visualized using an anti-human NFATc1-specific mAb (a-e). NFATc1 expression in untreated HPVECs (a) was detected in the cytoplasm, as evident by the diffuse staining throughout the cells. Nuclear localization of NFATc1 was evident in HPVECs treated with VEGF₁₆₅ (b). Pretreatment of HPVEC with 1 μM FK506 (c), 2 μM AAC789 (d), or 2 μM PTK787 (e) inhibited VEGF-induced nuclear translocation of NFATc1. VEGF-treated HPVEC incubated with isotype-matched control IgG (f).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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