|
Fig. 1 VEGF-induced NFATc1 nuclear translocation was blocked by the VEGF-R tyrosine kinase inhibitors. HPVEC grown for 24 hr in Endothelial Basal Media media with 10% FBS were either mock stimulated (a) or stimulated with 50 ng/ml VEGF165 (R&D Systems) for 30 min (b-f). Intracellular localization of NFATc1 was visualized using an anti-human NFATc1-specific mAb (a-e). NFATc1 expression in untreated HPVECs (a) was detected in the cytoplasm, as evident by the diffuse staining throughout the cells. Nuclear localization of NFATc1 was evident in HPVECs treated with VEGF165 (b). Pretreatment of HPVEC with 1 μM FK506 (c), 2 μM AAC789 (d), or 2 μM PTK787 (e) inhibited VEGF-induced nuclear translocation of NFATc1. VEGF-treated HPVEC incubated with isotype-matched control IgG (f).