ZFIN ID: ZDB-FIG-050630-4750
Thisse et al., 2001 - Expression of the zebrafish genome during embryogenesis (NIH R01 RR15402). ZFIN Direct Data Submission
Submitted By: F├╝rthauer, Maximilian, Thisse, Bernard, Thisse, Christine (Citing this work)
Thisse in situ hybridization protocol
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: 14-19 somites
Probe: cb110   Quality:

Fig. 4 At the 12 somite stage, expression is observed at the midbrain-hindbrain boundary, somites, posterior spinal cord, posterior floor plate, caudal tip of the notochord and cells ventral to it (endoderm?). Anteriorly, a strong staining is observed in the telencephalon, in head epidermis and in the optic stalk. A weak expression is observed in the ventral posterior diencephalon. At the 14 somite, expression appear in the retina as well as in the dorsal diencephalon. In the somites Fgf8 expression disappears from the posterior part and is restricted to the anterior cells lining the somitic furrow.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
fgf8a AB/TU standard conditions 14-19 somites basal plate midbrain region ISH
14-19 somites diencephalon ISH
14-19 somites floor plate diencephalic region ISH
14-19 somites floor plate neural rod ISH
14-19 somites floor plate rhombomere 1 ISH
14-19 somites floor plate rhombomere 2 ISH
14-19 somites floor plate rhombomere 3 ISH
14-19 somites floor plate rhombomere 4 ISH
14-19 somites floor plate rhombomere 5 ISH
14-19 somites floor plate rhombomere 6 ISH
14-19 somites floor plate rhombomere 7 ISH
14-19 somites floor plate rhombomere 8 ISH
14-19 somites floor plate telencephalic region ISH
14-19 somites heart primordium ISH
14-19 somites immature eye ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites neural tube ISH
14-19 somites notochord ISH
14-19 somites periderm ISH
14-19 somites somite ISH
14-19 somites tail bud ISH
14-19 somites telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available