FIGURE

Fig. S5

ID

ZDB-FIG-050609-12

Publication

Schäfer et al., 2005 - Medial floor plate formation in zebrafish consists of two phases and requires trunk-derived Midkine-a

Other Figures

All Figure Page

Back to All Figure Page

Fig. S5

Effect of mdka knock-down on notochord cell number. Notochord cells were counted in defined regions of the medial trunk (with respect to somite positions) of ten control embryos (a) and ten embryos injected with 9ng mdka morpholino (b). A highly significant increase in cell number was observed in morpholino injected embryos (Mann-Whitney U-test, n = 10, p < 0.001, Z = 3.711). Notochord cells were visualized by col2a1 expression and nuclear regions devoid of in-situ staining were marked (in red). For manual counting of notochord cells in whole mount preparations, one focal plane (focused on the MFP) was analyzed.

Expression Data

Gene:	col2a1a
Fish:	WT WT + MO1-mdka
Knockdown Reagent:	MO1-mdka
Anatomical Term:	notochord
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genes & Dev.