FIGURE

Fig. 5

ID
ZDB-FIG-050314-10
Publication
Crosnier et al., 2005 - Delta-Notch signalling controls commitment to a secretory fate in the zebrafish intestine
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Fig. 5

Intestinal phenotype of mib ta52b homozygotes. (A,B) 2F11 staining in the intestinal bulb, comparing three representative pairs of wild-type (A) and mib ta52b (B) specimens at 3 days. The vast majority of epithelial cells are switched to a secretory fate in mib ta52b. (C,D) At the same time, the number of cells expressing DeltaD (zdd-2 antibody, green, indicated by arrows in C) is greatly increased in the mutant. Membrane localization of DeltaD in mib ta52b reflects the failure of Mib-dependent Delta internalization. (E,F) 4E8 staining (green) shows deficit of absorptive cells in three-day mibta52b. (G,H) 2H9 staining (green) shows abnormal epithelial cell polarity in mib ta52b. (I,J) Staining of the basement membrane with 5F11 (green) is also fainter in mib ta52b than in wild-type. (K,L) At 4 days, many dying cells are seen in mib ta52b (L) but not in wild type (K). (A-L) TOPRO-3 nuclear stain is red.

Expression Data
Gene:
Fish:
Anatomical Term:
Stage: Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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