CRISPR

CRISPR1-mrpl4

ID

ZDB-CRISPR-260505-11

Name

CRISPR1-mrpl4

Previous Names

None

Target

mrpl4 (1)

Sequence

5' - GGGCTGGTGTCAAGTGAGCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ouc501	mrpl4
ouc508	mrpl4
ouc509	mrpl4
ouc510	mrpl4

Expression

Gene expression in Wild Types + CRISPR1-mrpl4

No data available

Phenotype

Phenotype resulting from CRISPR1-mrpl4

No data available

Phenotype of all Fish created by or utilizing CRISPR1-mrpl4

Phenotype	Fish	Conditions	Figures
intestine nfkbiab expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 3 from Li et al., 2026
intestine tgfb1a expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 3 from Li et al., 2026
enteroendocrine cell pyyb expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
yolk present, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
whole organism her15.1 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
intestine anterior region alpi.2 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestinal bulb notch1a expression decreased distribution, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
intestinal epithelial cell mitochondrion decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 4 from Li et al., 2026
whole organism decreased length, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
whole organism muc2.1 expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestine structure, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1, Fig. S1 from Li et al., 2026
intestine anterior region slc15a1b expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
swim bladder uninflated, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1, Fig. S1 from Li et al., 2026
whole organism hey1 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
intestinal bulb Notch signaling pathway decreased process quality, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
enteroendocrine cell decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
liver notch1a expression decreased distribution, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
proton motive force-driven mitochondrial ATP synthesis decreased process quality, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 4 from Li et al., 2026
liver hey1 expression decreased distribution, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
liver Notch signaling pathway decreased process quality, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
intestinal bulb chia.1 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestinal bulb hey1 expression decreased distribution, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
whole organism agr2 expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
whole organism decreased life span, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
intestine il1b expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 3 from Li et al., 2026
intestine anterior region fabp2 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestine lumen collapsed, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
goblet cell agr2 expression increased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestine inflammatory response increased occurrence, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 3 from Li et al., 2026
yolk increased size, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
whole organism notch1a expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 7 from Mo et al., 2023
whole organism mrpl4 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. EV5 from Mo et al., 2023
whole organism dead, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 1 from Li et al., 2026
intestinal bulb cell population proliferation decreased occurrence, abnormal	mrpl4^{ouc501/ouc501}	standard conditions	Fig. 2 from Li et al., 2026
intestine structure, abnormal	mrpl4^{ouc509/ouc509}	standard conditions	Fig. S1 from Li et al., 2026
swim bladder uninflated, abnormal	mrpl4^{ouc509/ouc509}	standard conditions	Fig. S1 from Li et al., 2026
intestine structure, abnormal	mrpl4^{ouc510/ouc510}	standard conditions	Fig. S1 from Li et al., 2026
swim bladder uninflated, abnormal	mrpl4^{ouc510/ouc510}	standard conditions	Fig. S1 from Li et al., 2026
intestinal bulb epithelium nucleus structure, abnormal	mrpl4^{ouc501/ouc501}; cc4Tg	standard conditions	Fig. 3 from Li et al., 2026
intestinal bulb epithelium tight junction structure, abnormal	mrpl4^{ouc501/ouc501}; cc4Tg	standard conditions	Fig. 3 from Li et al., 2026
intestine neutrophil infiltrative, abnormal	mrpl4^{ouc501/ouc501}; cc4Tg; i114Tg	standard conditions	Fig. 3 from Li et al., 2026
intestine neutrophil increased amount, abnormal	mrpl4^{ouc501/ouc501}; cc4Tg; i114Tg	standard conditions	Fig. 3 from Li et al., 2026
intestinal bulb chia.1 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}; cq89Tg	control	Fig. 5 from Li et al., 2026
intestine anterior region slc15a1b expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}; cq89Tg	control	Fig. 5 from Li et al., 2026
intestine morphology, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg	heat shock	Fig. 5 from Li et al., 2026
intestine anterior region alpi.2 expression amount, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg	heat shock	Fig. 5 from Li et al., 2026
intestine anterior region slc15a1b expression amount, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg	heat shock	Fig. 5 from Li et al., 2026
intestine anterior region fabp2 expression amount, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg	heat shock	Fig. 5 from Li et al., 2026
intestine anterior region alpi.2 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}; cq89Tg	control	Fig. 5 from Li et al., 2026
intestinal bulb chia.1 expression amount, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg	heat shock	Fig. 5 from Li et al., 2026
intestine anterior region fabp2 expression decreased amount, abnormal	mrpl4^{ouc501/ouc501}; cq89Tg	control	Fig. 5 from Li et al., 2026
intestine neutrophil amount, ameliorated	mrpl4^{ouc501/ouc501}; cq89Tg; i114Tg	heat shock	Fig. 5 from Li et al., 2026
intestine neutrophil increased amount, abnormal	mrpl4^{ouc501/ouc501}; cq89Tg; i114Tg	control	Fig. 5 from Li et al., 2026

Citations