CRISPR

CRISPR1-csde1

ID

ZDB-CRISPR-260126-4

Name

CRISPR1-csde1

Previous Names

None

Target

csde1 (1)

Sequence

5' - GGGAGTGGTTTGTGCTACCA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ioh3	csde1

Expression

Gene expression in Wild Types + CRISPR1-csde1

No data available

Phenotype

Phenotype resulting from CRISPR1-csde1

No data available

Phenotype of all Fish created by or utilizing CRISPR1-csde1

Phenotype	Fish	Conditions	Figures
whole organism rag1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
ventral wall of dorsal aorta hematopoietic multipotent progenitor cell runx1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. , Fig. 2. , Fig. 6. from Li et al., 2023
whole organism runx1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
hematopoietic multipotent progenitor cell runx1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
nucleate erythrocyte hbae1.1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
thymus lymphoid progenitor cell rag1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
whole organism myb expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
whole organism hbae1.1 expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
ventral wall of dorsal aorta hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. , Fig. 6. from Li et al., 2023
caudal hematopoietic tissue hematopoietic multipotent progenitor cell myb expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
thymus lymphoid progenitor cell rag1 expression decreased distribution, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
hematopoietic multipotent progenitor cell gfi1aa expression decreased amount, abnormal	csde1^ioh3/ioh3	standard conditions	Fig. 1. from Li et al., 2023
ventral wall of dorsal aorta hematopoietic multipotent progenitor cell EGFP expression decreased amount, abnormal	csde1^ioh3/ioh3; ioz1Tg/ioz1Tg; s896Tg/s896Tg	standard conditions	Fig. 1. from Li et al., 2023
caudal hematopoietic tissue hematopoietic multipotent progenitor cell EGFP expression decreased amount, abnormal	csde1^ioh3/ioh3; ioz1Tg/ioz1Tg; s896Tg/s896Tg	standard conditions	Fig. 1. from Li et al., 2023
Wnt signaling pathway decreased occurrence, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
endothelial cell differentiation decreased occurrence, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
stem cell development decreased occurrence, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
cell fate specification decreased occurrence, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
endothelial cell tcf7l2 expression decreased amount, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
cell population proliferation decreased occurrence, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
endothelial cell tcf3a expression decreased amount, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
endothelial cell vim expression decreased amount, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023
endothelial cell axin2 expression decreased amount, abnormal	csde1^ioh3/ioh3; s843Tg/s843Tg	standard conditions	Fig. 3. from Li et al., 2023

Citations