CRISPR

CRISPR4-col4a1

ID

ZDB-CRISPR-251124-3

Name

CRISPR4-col4a1

Previous Names

None

Target

col4a1 (1)

Sequence

5' - AGACTCACCGGAGGTCCATA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
umc104	col4a1

Expression

Gene expression in Wild Types + CRISPR4-col4a1

No data available

Phenotype

Phenotype resulting from CRISPR4-col4a1

No data available

Phenotype of all Fish created by or utilizing CRISPR4-col4a1

Phenotype	Fish	Conditions	Figures
brain hemorrhagic, abnormal	col4a1^{umc104/umc104}	standard conditions	Fig. 8 from Flatman et al., 2025
whole organism mmp13a.1 expression increased amount, abnormal	col4a1^{umc104/umc104}	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism col4a1 expression decreased amount, abnormal	col4a1^{umc104/umc104}	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism mmp9 expression increased amount, abnormal	col4a1^{umc104/umc104}	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism tgfb1a expression increased amount, abnormal	col4a1^{umc104/umc104}	standard conditions	Fig. 7 from Flatman et al., 2025
swimming behavior decreased process quality, abnormal	col4a1^{umc104/umc104}	control	Fig. 6 from Flatman et al., 2025
eye col4a1 expression decreased amount, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
brain hemorrhagic, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
whole organism mmp9 expression increased amount, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 4 from Flatman et al., 2025
whole organism col4a1 expression decreased amount, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
post-vent region col4a1 expression decreased amount, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
head col4a1 expression decreased amount, abnormal	WT + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
whole organism mmp13a.1 expression increased amount, abnormal	col4a1^umc104/+	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism mmp9 expression increased amount, abnormal	col4a1^umc104/+	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism col4a1 expression decreased amount, abnormal	col4a1^umc104/+	standard conditions	Fig. 7 from Flatman et al., 2025
whole organism tgfb1a expression increased amount, abnormal	col4a1^umc104/+	standard conditions	Fig. 7 from Flatman et al., 2025
swimming behavior decreased process quality, abnormal	col4a1^umc104/+	control	Fig. 6 from Flatman et al., 2025
mid cerebral vein absence of anatomical entity, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
brain hemorrhagic, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 3 from Flatman et al., 2025
basilar artery width, ameliorated	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	chemical treatment by environment: MMP9 inhibitor I	Fig. 4 from Flatman et al., 2025
forebrain vasculature amount, ameliorated	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	chemical treatment by environment: MMP9 inhibitor I	Fig. 4 from Flatman et al., 2025
primordial hindbrain channel width, ameliorated	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	chemical treatment by environment: MMP9 inhibitor I	Fig. 4 from Flatman et al., 2025
brain structure, ameliorated	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	chemical treatment by environment: MMP9 inhibitor I	Fig. 4 from Flatman et al., 2025
nasal vein absence of anatomical entity, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
brain vasculature structure, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
brain vasculature increased thickness, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
basilar artery increased width, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 3 , Fig. 4 from Flatman et al., 2025
brain vasculature kinked, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
primordial hindbrain channel increased width, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 3 , Fig. 4 from Flatman et al., 2025
primordial midbrain channel decreased distance primordial midbrain channel, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 3 from Flatman et al., 2025
brain vasculature EGFP expression spatial pattern, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 , Fig. 3 from Flatman et al., 2025
forebrain vasculature absence of anatomical entity, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 , Fig. 3 , Fig. 4 from Flatman et al., 2025
dorsal ciliary vein absence of anatomical entity, abnormal	s843Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 2 from Flatman et al., 2025
basilar artery increased width, abnormal	col4a1^umc104/+; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
primordial hindbrain channel increased width, abnormal	col4a1^umc104/+; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
brain hemorrhagic, abnormal	col4a1^{umc104/umc104}; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
brain structure, ameliorated	col4a1^{umc104/umc104}; s843Tg	chemical treatment by environment: atorvastatin	Fig. 5 from Flatman et al., 2025
basilar artery increased width, abnormal	col4a1^{umc104/umc104}; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
primordial hindbrain channel increased width, abnormal	col4a1^{umc104/umc104}; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
primordial midbrain channel decreased distance primordial midbrain channel, abnormal	col4a1^{umc104/umc104}; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
forebrain vasculature absence of anatomical entity, abnormal	col4a1^{umc104/umc104}; s843Tg	standard conditions	Fig. 5 from Flatman et al., 2025
brain hemorrhagic, abnormal	sd2Tg; y1Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025
brain nucleate erythrocyte DsRed expression spatial pattern, abnormal	sd2Tg; y1Tg + CRISPR4-col4a1 + CRISPR5-col4a1 + CRISPR6-col4a1 + CRISPR7-col4a1	control	Fig. 1 from Flatman et al., 2025

Citations