CRISPR

CRISPR1-ppp2r3b

ID

ZDB-CRISPR-230428-1

Name

CRISPR1-ppp2r3b

Previous Names

None

Target

Sequence

5' - GGAATGCTTTCACTTAAGGC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
u1001	ppp2r3b

Expression

Gene expression in Wild Types + CRISPR1-ppp2r3b

No data available

Phenotype

Phenotype resulting from CRISPR1-ppp2r3b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-ppp2r3b

Phenotype	Fish	Conditions	Figures
skeletal muscle mitochondrion decreased amount, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 11 from Seda et al., 2023
caudal fin curved lateral, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 5 from Seda et al., 2023
vertebra bone mineralization decreased process quality, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 8 from Seda et al., 2023
caudal vertebra curved ventral, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 5 from Seda et al., 2023
vertebra decreased mass density, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 9 from Seda et al., 2023
vertebral column rotational curvature, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 5 from Seda et al., 2023
vertebral column increased curvature, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 5 from Seda et al., 2023
precaudal vertebra curved ventral, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 5 from Seda et al., 2023
bone mineralization disrupted, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 9 from Seda et al., 2023
vertebra perforate, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 9 from Seda et al., 2023
skeletal muscle mitochondrion morphology, abnormal	ppp2r3b^u1001/u1001	standard conditions	Fig. 11 from Seda et al., 2023

Citations