CRISPR

CRISPR4-cep290

ID

ZDB-CRISPR-230307-2

Name

CRISPR4-cep290

Previous Names

None

Target

Sequence

5' - TCGCCAACTGGAGAGTCACC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
uab18	cep290

Expression

Gene expression in Wild Types + CRISPR4-cep290

No data available

Phenotype

Phenotype resulting from CRISPR4-cep290

No data available

Phenotype of all Fish created by or utilizing CRISPR4-cep290

Phenotype	Fish	Conditions	Figures
trunk increased curvature, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
male organism male sterile, abnormal	cep290^uab18/uab18 (AB)	standard conditions	text only from Wang et al., 2022
female organism female sterile, abnormal	cep290^uab18/uab18 (AB)	standard conditions	text only from Wang et al., 2022
posterior lateral line neuromast amount, ameliorated	cep290^uab18/uab18 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
retinal outer nuclear layer cell population proliferation increased occurrence, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 4. from Wang et al., 2022
anterior lateral line neuromast amount, ameliorated	cep290^uab18/uab18 (AB)	chemical treatment by environment: neomycin	Fig. 3. from Wang et al., 2022
vertebral column increased curvature, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 1. , Fig. 2. from Wang et al., 2022
vertebral column rotational curvature, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 1. , Fig. 2. from Wang et al., 2022
post-vent region curved ventral, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 1. from Wang et al., 2022
retina inflammatory response increased occurrence, abnormal	cep290^uab18/uab18 (AB)	standard conditions	Fig. 4. from Wang et al., 2022

Citations