CRISPR

CRISPR1-dcst1

ID
ZDB-CRISPR-220111-1
Name
CRISPR1-dcst1
Previous Names
None
Target
Sequence
5' - GGTTAAACGCACTTGAATGG - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
None
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
vbc14 dcst1
vbc16b dcst2
Expression
Gene expression in Wild Types + CRISPR1-dcst1
No data available
Phenotype
Phenotype resulting from CRISPR1-dcst1
No data available
Phenotype of all Fish created by or utilizing CRISPR1-dcst1
Phenotype Fish Conditions Figures
male organism decreased male fertility, abnormal dcst1vbc14/vbc14 (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm sperm head dcst2 expression decreased distribution, abnormal dcst1vbc14/vbc14 (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm dcst2 expression absent, abnormal dcst1vbc14/vbc14 (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm dcst1 expression absent, abnormal dcst1vbc14/vbc14 (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm sperm head dcst2 expression spatial pattern, abnormal dcst1vbc14/vbc14 (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
male organism male sterile, abnormal dcst1vbc16a/vbc16a; dcst2vbc16b/vbc16b (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm sperm head plasma membrane dcst2 expression absent, abnormal dcst1vbc16a/vbc16a; dcst2vbc16b/vbc16b (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
fusion of sperm to egg plasma membrane involved in single fertilization disrupted, abnormal dcst1vbc16a/vbc16a; dcst2vbc16b/vbc16b (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm dcst2 expression absent, abnormal dcst1vbc16a/vbc16a; dcst2vbc16b/vbc16b (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
sperm dcst1 expression absent, abnormal dcst1vbc16a/vbc16a; dcst2vbc16b/vbc16b (AB/TL) standard conditions Fig. 5 with image from Noda et al., 2022
Citations
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