CRISPR

CRISPR1-cdx1b

ID

ZDB-CRISPR-210913-1

Name

CRISPR1-cdx1b

Previous Names

None

Target

Sequence

5' - GTTCCAGCTGCCCGTC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 5' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
as39	cdx1b

Expression

Gene expression in Wild Types + CRISPR1-cdx1b

No data available

Phenotype

Phenotype resulting from CRISPR1-cdx1b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-cdx1b

Phenotype	Fish	Conditions	Figures
determination of left/right asymmetry in nervous system disrupted, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 4 from Wu et al., 2020
diencephalon dorsal region ndr2 expression absent, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 3 from Wu et al., 2020
endodermal cell sox17 expression decreased distribution, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 5 from Wu et al., 2020
endodermal cell foxa2 expression decreased distribution, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 5 from Wu et al., 2020
heart rudiment lft1 expression absent, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 3 from Wu et al., 2020
parapineal organ gfi1ab expression spatial pattern, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 4 from Wu et al., 2020
habenula right side kctd12.1 expression increased amount, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 4 from Wu et al., 2020
diencephalon dorsal region pitx2 expression mislocalised, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 2 from Wu et al., 2020
diencephalon dorsal region pitx2 expression absent, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 3 from Wu et al., 2020
diencephalon dorsal region lft1 expression absent, abnormal	cdx1b^as39/as39 (AB)	standard conditions	Fig. 3 from Wu et al., 2020

Citations