CRISPR

CRISPR1-stag2b

ID

ZDB-CRISPR-210819-3

Name

CRISPR1-stag2b

Previous Names

None

Target

stag2b (1)

Sequence

5' - GGCCCTGGAGAGAAGGGAAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "AGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
nz207	stag2b

Expression

Gene expression in Wild Types + CRISPR1-stag2b

No data available

Phenotype

Phenotype resulting from CRISPR1-stag2b

No data available

Phenotype of all Fish created by or utilizing CRISPR1-stag2b

Phenotype	Fish	Conditions	Figures
anterior lateral mesoderm tal1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 6 from Ketharnathan et al., 2020
posterior lateral mesoderm anterior region tal1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 4 from Ketharnathan et al., 2020
whole organism tal1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 4 from Ketharnathan et al., 2020
posterior lateral mesoderm fli1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 5 from Ketharnathan et al., 2020
posterior lateral mesoderm runx1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 4 from Ketharnathan et al., 2020
posterior lateral mesoderm tal1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 5 from Ketharnathan et al., 2020
swim bladder swim bladder inflation delayed, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 2 from Ketharnathan et al., 2020
anterior lateral mesoderm spi1b expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 6 from Ketharnathan et al., 2020
anterior lateral mesoderm runx1 expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 6 from Ketharnathan et al., 2020
caudal fin melanocyte displaced, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 2 from Ketharnathan et al., 2020
posterior lateral mesoderm gata1a expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 5 from Ketharnathan et al., 2020
erythroid lineage cell gata1a expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 3 from Ketharnathan et al., 2020
whole organism gata1a expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 3 , Figure 4 from Ketharnathan et al., 2020
anterior neural rod pax2a expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 5 from Ketharnathan et al., 2020
erythroid lineage cell decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 3 from Ketharnathan et al., 2020
whole organism stag2b expression decreased amount, abnormal	stag2b^nz207/nz207 (WIK)	standard conditions	Figure 2 from Ketharnathan et al., 2020
erythroid lineage cell gata1a expression decreased amount, abnormal	stag2b^nz207/+ (WIK)	standard conditions	Figure 3 from Ketharnathan et al., 2020
erythroid lineage cell decreased amount, abnormal	stag2b^nz207/+ (WIK)	standard conditions	Figure 3 from Ketharnathan et al., 2020
midbrain mCherry expression increased amount, abnormal	stag2b^nz207/nz207; ia5Tg/+	chemical treatment by environment: lithium chloride	Figure 7. from Chin et al., 2020
midbrain Wnt signaling pathway increased occurrence, abnormal	stag2b^nz207/nz207; ia5Tg/+	standard conditions	Figure 7. from Chin et al., 2020
midbrain mCherry expression increased amount, abnormal	stag2b^nz207/nz207; ia5Tg/+	standard conditions	Figure 7. from Chin et al., 2020

Citations