CRISPR

CRISPR2-piezo1

ID

ZDB-CRISPR-210518-3

Name

CRISPR2-piezo1

Previous Names

None

Target

piezo1 (1)

Sequence

5' - GCTCAGTGTGGTCAACCCGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ion89d	piezo1

Expression

Gene expression in Wild Types + CRISPR2-piezo1

No data available

Phenotype

Phenotype resulting from CRISPR2-piezo1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-piezo1

Phenotype	Fish	Conditions	Figures
calcium-mediated signaling decreased process quality, abnormal	piezo1^{ion89d/ion89d}; ion20dTg; s896Tg	standard conditions	Fig. 4 from Liu et al., 2020
brain vasculature Notch signaling pathway normal rate, ameliorated	piezo1^{ion89d/ion89d}; mw43Tg/mw43Tg; um13Tg/um13Tg	chemical treatment by environment: yoda 1	Fig. 4 from Zi et al., 2024
brain blood vessel GFP expression amount, ameliorated	piezo1^{ion89d/ion89d}; mw43Tg/mw43Tg; um13Tg/um13Tg	chemical treatment by environment: yoda 1	Fig. 4 from Zi et al., 2024
response to mechanical stimulus absent process, abnormal	piezo1^{ion89d/ion89d}; s896Tg	mechanical stress, physical alteration: endothelial tip cell	Fig. 5 from Liu et al., 2020
calcium-mediated signaling absent process, abnormal	piezo1^{ion89d/ion89d}; s896Tg	mechanical stress, physical alteration: endothelial tip cell	Fig. 5 from Liu et al., 2020
calcium-mediated signaling disrupted, abnormal	piezo1^{ion89d/ion89d}; s896Tg	mechanical stress, physical alteration: endothelial tip cell	Fig. 5 from Liu et al., 2020
cranial vasculature spatial pattern, abnormal	piezo1^ion89d; s843Tg	standard conditions	Fig. 6 from Liu et al., 2020
cranial blood vessel bent, abnormal	piezo1^ion89d; s843Tg	standard conditions	Fig. 6 from Liu et al., 2020
sprouting angiogenesis disrupted, abnormal	piezo1^ion89d; s843Tg	standard conditions	Fig. 6 from Liu et al., 2020
cranial blood vessel increased branchiness, abnormal	piezo1^ion89d; s843Tg	standard conditions	Fig. 6 from Liu et al., 2020
pericyte cell division decreased frequency, abnormal	piezo1^{ion89d/ion89d}; pdgfrb^{ion33dTg/ion33dTg}; c369Tg/c369Tg; um13Tg/um13Tg	standard conditions	Fig. 2 from Zi et al., 2024
midbrain blood vessel DsRedx expression increased amount, abnormal	piezo1^{ion89d/ion89d}; pdgfrb^{ion33dTg/ion33dTg}; c369Tg/c369Tg; um13Tg/um13Tg	standard conditions	Fig. 3 from Zi et al., 2024
brain vasculature pericyte GFP expression decreased amount, abnormal	piezo1^{ion89d/ion89d}; pdgfrb^{ion33dTg/ion33dTg}; c369Tg/c369Tg; um13Tg/um13Tg	standard conditions	Fig. 2 from Zi et al., 2024
brain vasculature pericyte GFP expression amount, ameliorated	piezo1^{ion89d/ion89d}; pdgfrb^{ion33dTg/ion33dTg}; c369Tg/c369Tg; um13Tg/um13Tg	chemical treatment by environment: Epinephrine bitartrate	Fig. 2 from Zi et al., 2024
brain vasculature pericyte GFP expression amount, ameliorated	piezo1^{ion89d/ion89d}; pdgfrb^{ion33dTg/ion33dTg}; c369Tg/c369Tg; um13Tg/um13Tg	chemical treatment by environment: diacetylmonoxime	Fig. 2 from Zi et al., 2024

Citations