CRISPR

CRISPR1-prdm16

ID

ZDB-CRISPR-201203-13

Name

CRISPR1-prdm16

Previous Names

None

Target

prdm16 (1)

Sequence

5' - TGCAACGAGGACTTCACTCCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
co1006	prdm16
co1014	prdm16

Expression

Gene expression in Wild Types + CRISPR1-prdm16

No data available

Phenotype

Phenotype resulting from CRISPR1-prdm16

No data available

Phenotype of all Fish created by or utilizing CRISPR1-prdm16

Phenotype	Fish	Conditions	Figures
pharyngeal arch cartilage col2a1a expression decreased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
pharyngeal arch 3-7 prdm1a expression increased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
chondrocranium chondrocyte disorganized, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 1 from Shull et al., 2022
opercle decreased size, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
chondrocranium cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 1 , Fig. 6 from Shull et al., 2022
cranial cartilage col2a1a expression decreased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
ceratobranchial 5 bone decreased size, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
chondrocranium cartilage cartilage morphogenesis decreased process quality, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 1 from Shull et al., 2022
trabecula cranii decreased distance trabecula cranii, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
pectoral fin bud prdm1a expression increased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
parasphenoid decreased length, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
parasphenoid bone mineralization decreased occurrence, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
opercle bone mineralization decreased occurrence, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
pharyngeal arch runx2b expression decreased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
head mecom expression decreased amount, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
eye prdm1a expression increased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
cranium runx2b expression decreased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
head prdm1a expression increased amount, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial 5 bone bone mineralization decreased occurrence, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
chondrocranium mediolateral intercalation normal process quality, ameliorated	prdm16^{co1006/co1006}	chemical treatment by environment: Wnt signalling activator	Fig. 6 from Shull et al., 2022
hatching gland prdm1a expression increased distribution, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 3 from Shull et al., 2020
chondrocranium mediolateral intercalation decreased process quality, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 1 , Fig. 6 from Shull et al., 2022
ceratobranchial cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}	standard conditions	Fig. 2 from Shull et al., 2020
cranial neural crest cell pygo1 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
chondrocyte nucleus Ab5-ctnnb1 labeling decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 4 from Shull et al., 2022
Wnt signalosome assembly decreased rate, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell smarca4a expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell pygo2 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell tcf7 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell apc expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell jun expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell fzd3b expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest chromatin remodeling decreased process quality, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 5 from Shull et al., 2022
cranial neural crest cell lef1 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell fosab expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell bcl9 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell ctnnb1 expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
cranial neural crest cell dvl3a expression decreased amount, abnormal	prdm16^{co1006/co1006}; ba2Tg/ba2Tg	standard conditions	Fig. 3 from Shull et al., 2022
pharyngeal arch Wnt signalosome mCherry expression decreased amount, abnormal	prdm16^{co1006/co1006}; ia5Tg/ia5Tg; y1Tg/y1Tg	standard conditions	Fig. 4 from Shull et al., 2022
chondrocranium mediolateral intercalation normal process quality, ameliorated	prdm16^{co1006/co1006}; mecom^{co1005/co1005}	standard conditions	Fig. 6 from Shull et al., 2022
chondrocranium cartilage normal amount, ameliorated	prdm16^{co1006/co1006}; mecom^{co1005/co1005}	standard conditions	Fig. 6 from Shull et al., 2022
palatoquadrate cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^co1006/+; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^co1006/+; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/+; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^co1005/+	standard conditions	Fig. 3 from Shull et al., 2020
palatoquadrate cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ethmoid cartilage split, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage decreased distance palatoquadrate cartilage, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
ceratobranchial cartilage aplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020
Meckel's cartilage aplastic/hypoplastic, abnormal	prdm16^{co1006/co1006}; prdm1a^m805/m805; mecom^{co1005/co1005}	standard conditions	Fig. 3 from Shull et al., 2020

Citations