CRISPR

CRISPR2-sspo

ID

ZDB-CRISPR-201019-1

Name

CRISPR2-sspo

Previous Names

CRISPR2-scospondin

Target

sspo (1)

Sequence

5' - AGTGTACCAGCTGCCAGGGT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "GGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ut24Tg	sspo

Expression

Gene expression in Wild Types + CRISPR2-sspo

No data available

Phenotype

Phenotype resulting from CRISPR2-sspo

No data available

Phenotype of all Fish created by or utilizing CRISPR2-sspo

Phenotype	Fish	Conditions	Figures
whole organism axis curved, abnormal	sspo^ut24Tg/+	standard conditions	Fig. 4 from Troutwine et al., 2020
central canal EGFP expression increased distribution, abnormal	cfap298^tm304/tm304; sspo^ut24Tg	standard conditions	Figure 5. from Bearce et al., 2022
central canal EGFP expression spatial pattern, abnormal	cfap298^tm304/tm304; sspo^ut24Tg	standard conditions	Figure 5. from Bearce et al., 2022
central canal lacks all parts of type Reissner's fiber, abnormal	cfap298^tm304/tm304; sspo^ut24Tg	standard conditions	Figure 5. from Bearce et al., 2022
Reissner's fiber disassembled, abnormal	dnah10^ut28/ut28; sspo^ut24Tg/+ (AB)	standard conditions	Fig. 4 from Wang et al., 2021
cilium movement disrupted, abnormal	dnah10^ut28/ut28; sspo^ut24Tg/+ (AB)	standard conditions	Fig. 4 from Wang et al., 2021
Reissner's fiber EGFP expression absent, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
post-vent region increased curvature, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
Reissner's fiber absence of anatomical entity, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
floor plate basal side EGFP expression spatial pattern, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
post-vent region curved, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
floor plate basal side EGFP expression mislocalised, abnormal	dpy19l1l^nkz5/nkz5; sspo^ut24Tg/+ (AB/TU)	standard conditions	Fig. 4 from Usami et al., 2026
whole organism axis curved, abnormal	druk^dut26Et/+; sspo^ut24Tg/+	standard conditions	Fig. 4 from Troutwine et al., 2020

Citations