CRISPR

CRISPR1-pcyt2

ID

ZDB-CRISPR-200604-5

Name

CRISPR1-pcyt2

Previous Names

None

Target

Sequence

5' - GATACGGGCCATCAAGTGGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM site was "TGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

No data available

Expression

Gene expression in Wild Types + CRISPR1-pcyt2

Expressed Gene	Anatomy	Figures
pcyt2	whole organism	Fig. 4 from Vaz et al., 2019

Phenotype

Phenotype resulting from CRISPR1-pcyt2

Phenotype	Fish	Figures
skeletal muscle decreased mass, abnormal	AB + CRISPR1-pcyt2	Fig. S4 from Cikes et al., 2023
skeletal muscle cell myofibril decreased size, abnormal	AB + CRISPR1-pcyt2	Fig. 1 from Cikes et al., 2023
whole organism pcyt2 expression decreased amount, abnormal	AB + CRISPR1-pcyt2	Fig. 4 from Vaz et al., 2019
whole organism decreased length, abnormal	AB + CRISPR1-pcyt2	Fig. 1 from Cikes et al., 2023
whole organism decreased life span, abnormal	AB + CRISPR1-pcyt2	Fig. 4 from Vaz et al., 2019

Phenotype of all Fish created by or utilizing CRISPR1-pcyt2

Phenotype	Fish	Conditions	Figures
skeletal muscle decreased mass, abnormal	AB + CRISPR1-pcyt2	control	Fig. S4 from Cikes et al., 2023
whole organism decreased length, abnormal	AB + CRISPR1-pcyt2	control	Fig. 1 from Cikes et al., 2023
whole organism pcyt2 expression decreased amount, abnormal	AB + CRISPR1-pcyt2	standard conditions	Fig. 4 from Vaz et al., 2019
skeletal muscle cell myofibril decreased size, abnormal	AB + CRISPR1-pcyt2	control	Fig. 1 from Cikes et al., 2023
whole organism decreased life span, abnormal	AB + CRISPR1-pcyt2	standard conditions	Fig. 4 from Vaz et al., 2019

Citations