CRISPR

CRISPR1-dre-mir-182

ID
ZDB-CRISPR-191202-3
Name
CRISPR1-dre-mir-182
Previous Names
  • CRISPR1-mir182
Target
Sequence
5' - GGGGACAAAAGGTTCTCTGA - 3'
ZFIN BLAST NCBI BLAST Ensembl UCSC BLAT
Disclaimer
Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.
Note
The first two 'G's were added to facilitate T7-dependent transcription.
Genome Resources
None
Target Location
Constructs
No data available
Genomic Features
Genomic Feature Affected Genomic Regions
Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78 dre-mir-96, dre-mir-182, dre-mir-183
Df(Chr4:dre-mir-183,dre-mir-182)lri81 dre-mir-182, dre-mir-183
lri61 dre-mir-182
lri79a dre-mir-96
lri79b dre-mir-182
Expression
Gene expression in Wild Types + CRISPR1-dre-mir-182
No data available
Phenotype
Phenotype resulting from CRISPR1-dre-mir-182
No data available
Phenotype of all Fish created by or utilizing CRISPR1-dre-mir-182
Phenotype Fish Conditions Figures
neuromast has fewer parts of type neuromast hair cell, abnormal Df(Chr4:dre-mir-183,dre-mir-182)lri81/lri81 chemical treatment by environment: gentamycin Fig. 7 with image from Fogerty et al., 2019
retina dre-mir-183 expression decreased amount, abnormal Df(Chr4:dre-mir-183,dre-mir-182)lri81/lri81 standard conditions Fig. 3 with image from Fogerty et al., 2019
retina dre-mir-182 expression decreased amount, abnormal Df(Chr4:dre-mir-183,dre-mir-182)lri81/lri81 standard conditions Fig. 3 with image from Fogerty et al., 2019
retina dre-mir-96 expression decreased amount, abnormal dre-mir-96lri79a/lri79a standard conditions Fig. 3 with image from Fogerty et al., 2019
neuromast has fewer parts of type neuromast hair cell, abnormal dre-mir-96lri79a/lri79a chemical treatment by environment: gentamycin Fig. 7 with image from Fogerty et al., 2019
retina dre-mir-182 expression decreased amount, abnormal dre-mir-96lri79a/lri79a standard conditions Fig. 3 with image from Fogerty et al., 2019
neuromast has fewer parts of type neuromast hair cell, abnormal dre-mir-182lri61/lri61 chemical treatment by environment: gentamycin Fig. 7 with image from Fogerty et al., 2019
retina dre-mir-182 expression decreased amount, abnormal dre-mir-182lri61/lri61 standard conditions Fig. 3 with image from Fogerty et al., 2019
neuromast hair cell stereocilium maintenance decreased process quality, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 7 with image from Fogerty et al., 2019
retina dre-mir-96 expression absent, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 3 with image from Fogerty et al., 2019
whole organism semi-viable, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 6 with image from Fogerty et al., 2019
neuromast has fewer parts of type neuromast hair cell, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 chemical treatment by environment: gentamycin Fig. 7 with image from Fogerty et al., 2019
retinal cone cell photoreceptor inner segment decreased length, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 4 with image from Fogerty et al., 2019
retina dre-mir-183 expression absent, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 3 with image from Fogerty et al., 2019
retina dre-mir-182 expression decreased amount, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 3 with image from Fogerty et al., 2019
neuromast hair cell detection of mechanical stimulus involved in sensory perception of sound decreased process quality, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 8 with image from Fogerty et al., 2019
neuromast hair cell stereocilium bundle malformed, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 7 with image from Fogerty et al., 2019
neuromast has fewer parts of type neuromast hair cell stereocilium bundle, abnormal Df(Chr4:dre-mir183,dre-mir096,dre-mir-182)lri78/lri78 standard conditions Fig. 8 with image from Fogerty et al., 2019
Citations
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