CRISPR

CRISPR1-sema3fa

ID

ZDB-CRISPR-190204-1

Name

CRISPR1-sema3fa

Previous Names

None

Target

sema3fa (1)

Sequence

5' - GAAGACTCGTGGAACAGAGG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ca304	sema3fa
zf3897	sema3fa

Expression

Gene expression in Wild Types + CRISPR1-sema3fa

No data available

Phenotype

Phenotype resulting from CRISPR1-sema3fa

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sema3fa

Phenotype	Fish	Conditions	Figures
whole organism sema3fa expression decreased amount, abnormal	sema3fa^ca304/ca304	standard conditions	Fig. 1 from Halabi et al., 2021
retinal neural layer deformed, abnormal	sema3fa^ca304/ca304	standard conditions	Fig. 4 from Halabi et al., 2021
optic choroid vascular plexus capillary displaced to retinal outer nuclear layer, abnormal	sema3fa^ca304/ca304	standard conditions	Fig. 4 from Halabi et al., 2021
whole organism sema3fa expression absent, abnormal	sema3fa^ca304/ca304	standard conditions	Fig. 1 from Halabi et al., 2021
optic choroid vascular plexus capillary increased permeability, abnormal	sema3fa^ca304/ca304	standard conditions	Fig. 4 from Halabi et al., 2021
whole organism increased length, abnormal	WIK + CRISPR1-sema3fa + CRISPR2-sema3fa	control	Fig. S3 from van der Klaauw et al., 2019
whole organism increased weight, abnormal	WIK + CRISPR1-sema3fa + CRISPR2-sema3fa	control	Fig. S3 from van der Klaauw et al., 2019
hyaloid vessel increased branchiness, abnormal	sema3fa^ca304/+; ci5Tg	standard conditions	Fig. 2 from Halabi et al., 2021
hyaloid vessel blood vessel remodeling decreased occurrence, abnormal	sema3fa^ca304/+; ci5Tg	standard conditions	Fig. 2 from Halabi et al., 2021
optic choroid vascular plexus increased size, abnormal	sema3fa^ca304/+; ci5Tg	standard conditions	Fig. 4 from Halabi et al., 2021
optic choroid vascular plexus capillary increased permeability, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 5 from Halabi et al., 2021
hyaloid vessel increased branchiness, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 2 , Fig. 3 from Halabi et al., 2021
hyaloid vessel peripheral region has extra parts of type hyaloid capillaries filopodium, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 3 from Halabi et al., 2021
hyaloid vessel decreased length, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 3 from Halabi et al., 2021
hyaloid vessel blood vessel remodeling decreased occurrence, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 2 , Fig. 3 from Halabi et al., 2021
hyaloid vessel decreased width, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 3 from Halabi et al., 2021
optic choroid vascular plexus increased size, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 4 from Halabi et al., 2021
optic choroid vascular plexus capillary displaced to photoreceptor outer segment layer, abnormal	sema3fa^ca304/ca304; ci5Tg	standard conditions	Fig. 5 from Halabi et al., 2021

Citations