ZFIN ID: ZDB-FIG-210324-50
Halabi et al., 2021 - Semaphorin 3fa Controls Ocular Vascularization From the Embryo Through to the Adult. Investigative ophthalmology & visual science   62:21 Full text @ Invest. Ophthalmol. Vis. Sci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 2 Sema3fa is required to refine and limit retinal vessel branching in the larval eye. Live imaging of vessels (white) in Tg(kdrl:mCherry) WT (A,C,E,E’) and sema3faca304 heterozygous (G,G’), and homozygous (B,D,F,F’) eyes during retinal intraocular vascular development acquired by confocal microscopy. (A, B) In lateral views of WT (A) and sema3faca304 homozygous embryos, the hyaloid artery (endothelial cells labeled by mCherry) contacts the lens (asterisk) of the retina by 24 hpf (N = 1 WT n = 3; sema3faca304 n = 4). (C, D) Ventral views of WT (C: N = 1, n = 4) and sema3faca304 (D: N = 1, n = 4) eyes reveal that the hyaloid artery (arrowhead) forms a vascular plexus network around the lens by 72 hpf. Also evident is the nasal ciliary artery (arrows). (E–G) The WT intraocular hyaloid vessel (E’ is a magnified view of the boxed area in E) undergoes remodeling between 4-5 dpf to simplify the network (Hartsock et al., 2014). At 4 dpf, the numbers of vessel crossovers (arrows) are increased in sema3fa heterozygous (G,G’: N = 1, n = 2/2) and homozygous (F,F’: N = 4, n = 12/12) mutant embryos as compared to WT siblings (E, E’: N = 4, n = 10/11 show refinement). Scale bar: 100 µm; 50 µm for E’–G’.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
mCherry ci5Tg standard conditions Prim-5 hyaloid artery blood vessel endothelial cell IFL
Protruding-mouth hyaloid artery blood vessel endothelial cell IFL
Protruding-mouth nasal ciliary artery blood vessel endothelial cell IFL
Day 4 hyaloid vessel blood vessel endothelial cell IFL
sema3faca304/+; ci5Tg standard conditions Day 4 hyaloid vessel blood vessel endothelial cell IFL
sema3faca304/ca304; ci5Tg standard conditions Prim-5 hyaloid artery blood vessel endothelial cell IFL
Protruding-mouth hyaloid artery blood vessel endothelial cell IFL
Protruding-mouth nasal ciliary artery blood vessel endothelial cell IFL
Day 4 hyaloid vessel blood vessel endothelial cell IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
sema3faca304/+; ci5Tg standard conditions Day 4 hyaloid vessel blood vessel remodeling decreased occurrence, abnormal
Day 4 hyaloid vessel increased branchiness, abnormal
sema3faca304/ca304; ci5Tg standard conditions Prim-5 hyaloid vessel morphology, normal
Prim-5 nasal ciliary artery morphology, normal
Protruding-mouth hyaloid vessel morphology, normal
Protruding-mouth nasal ciliary artery morphology, normal
Day 4 hyaloid vessel blood vessel remodeling decreased occurrence, abnormal
Day 4 hyaloid vessel increased branchiness, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Investigative ophthalmology & visual science for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Invest. Ophthalmol. Vis. Sci.