CRISPR

CRISPR1-sspo

ID

ZDB-CRISPR-181113-2

Name

CRISPR1-sspo

Previous Names

CRISPR1-scospondin

Target

sspo (1)

Sequence

5' - GGCTGGATGTGGAGCGCATG - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
icm13	sspo
icm15	sspo

Expression

Gene expression in Wild Types + CRISPR1-sspo

No data available

Phenotype

Phenotype resulting from CRISPR1-sspo

No data available

Phenotype of all Fish created by or utilizing CRISPR1-sspo

Phenotype	Fish	Conditions	Figures
cerebrospinal fluid contacting neuron urp2 expression amount, ameliorated	sspo^icm13/icm13	chemical treatment by environment: (R)-adrenaline	Fig. 3 from Lu et al., 2020
post-vent region curved ventral, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
cerebrospinal fluid contacting neuron urp1 expression decreased amount, abnormal	sspo^icm13/icm13	standard conditions	Fig. 3 from Lu et al., 2020
vertebral column increased curvature, abnormal	sspo^icm13/icm13	standard conditions	Fig. 2 from Lu et al., 2020
third ventricle lacks all parts of type Reissner's fiber, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
trunk curvature, ameliorated	sspo^icm13/icm13	chemical treatment by environment: (R)-adrenaline	Fig. 5 from Lu et al., 2020
trunk curved, abnormal	sspo^icm13/icm13	standard conditions	Fig. 2 from Lu et al., 2020
post-vent region curved, abnormal	sspo^icm13/icm13	standard conditions	Fig. 2 from Lu et al., 2020
cerebrospinal fluid contacting neuron urp1 expression amount, ameliorated	sspo^icm13/icm13	chemical treatment by environment: (R)-adrenaline	Fig. 3 from Lu et al., 2020
vertebral column rotational curvature, abnormal	sspo^icm13/icm13	standard conditions	Fig. 2 from Lu et al., 2020
ventricular system lacks all parts of type Reissner's fiber, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
whole organism urp1 expression decreased amount, abnormal	sspo^icm13/icm13	chemical treatment by environment: (R)-adrenaline	Fig. 4 from Lu et al., 2020
vertebral column bent, abnormal	sspo^icm13/icm13	standard conditions	Fig. 2 from Lu et al., 2020
Reissner's fiber Ab1-RF labeling absent, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
subcommissural organ Ab1-RF labeling absent, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
trunk curved ventral, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Lu et al., 2020
central canal lacks all parts of type Reissner's fiber, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
whole organism urp2 expression decreased amount, abnormal	sspo^icm13/icm13	chemical treatment by environment: (R)-adrenaline	Fig. 4 from Lu et al., 2020
floor plate Ab1-RF labeling absent, abnormal	sspo^icm13/icm13	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
cerebrospinal fluid contacting neuron urp2 expression decreased amount, abnormal	sspo^icm13/icm13	standard conditions	Fig. 3 from Lu et al., 2020
post-vent region curved ventral, abnormal	sspo^icm15/icm15	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
third ventricle lacks all parts of type Reissner's fiber, abnormal	sspo^icm15/icm15	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
ventricular system lacks all parts of type Reissner's fiber, abnormal	sspo^icm15/icm15	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
Reissner's fiber Ab1-RF labeling absent, abnormal	sspo^icm15/icm15	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
central canal lacks all parts of type Reissner's fiber, abnormal	sspo^icm15/icm15	standard conditions	Fig. 1 from Cantaut-Belarif et al., 2018
cerebrospinal fluid contacting neuron response to mechanical stimulus process quality, abnormal	sspo^icm15/icm15; icm07Tg	control	Fig. 3 from Orts-Del'Immagine et al., 2020
cerebrospinal fluid contacting neuron response to mechanical stimulus decreased occurrence, abnormal	sspo^icm15/icm15; icm07Tg	control	Fig. 3 from Orts-Del'Immagine et al., 2020

Citations