CRISPR

CRISPR10-ltk

ID

ZDB-CRISPR-181015-10

Name

CRISPR10-ltk

Previous Names

None

Target

ltk (1)

Sequence

5' - GGGAGTTGGAGATGACAAAA - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The first two "G"s were added to the 5' end to enhance binding.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
ya347	ltk

Expression

Gene expression in Wild Types + CRISPR10-ltk

No data available

Phenotype

Phenotype resulting from CRISPR10-ltk

No data available

Phenotype of all Fish created by or utilizing CRISPR10-ltk

Phenotype	Fish	Conditions	Figures
eye lacks all parts of type iridophore, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 2, Fig. 4 from Mo et al., 2017
caudal fin lacks all parts of type iridophore, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 2 from Mo et al., 2017
melanophore stripe morphology, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
trunk lacks all parts of type iridophore, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
whole organism ltk expression decreased amount, abnormal	ltk^ya347/ya347 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
eye lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 2, Fig. 4 from Mo et al., 2017
whole organism ltk expression decreased amount, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
trunk lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
caudal fin lacks all parts of type iridophore, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 2 from Mo et al., 2017
whole organism alk expression decreased amount, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 1 from Mo et al., 2017
iridophore differentiation decreased occurrence, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017
melanophore stripe morphology, abnormal	alk^ya343/ya343; ltk^ya347/ya347 (AB)	standard conditions	Fig. 4 from Mo et al., 2017

Citations