This material is from the 4th edition of The Zebrafish Book. The 5th edition is available in print and within the ZFIN Protocol Wiki.


Preparing subbed slides for sections

(Source: K. Larison)

For most purposes (e.g. immunohistochemistry and in situ hybridization) Vectabond (Vector Laboratories, 800-227-6666) works well for coating slides.

For epon and paraffin embedded sections, gelatin subbed slides may be more appropriate. To prepare subbed slides:

1. Soak new microslides overnight in acid cleaning solution (eg.chromium sulfuric acid.) A strong solution of Micro can be substituted.

2. Wash slides thoroughly in running tap water and rinse in several changes of dH2O.

3. Transfer slides into slide carriers and dip three times in the gelatin solution.

4.Tto avoid streaking, rap the slide carrier vigorously on a paper towel on the counter top following the last immersion.

5. Drain well and dry overnight at 40C.

Gelatin subbing solution:

0.5% gelatin
0.05% CrK(SO4)x12H2O.

The Zebrafish Book