Research
General Information
ZIRC
ZFIN: Mapping Panel: Heat Shock
ZFIN ID: ZDB-REFCROSS-000320-1

Meiotic Panel: Heat Shock (HS)

Description: The Talbot and Postlethwait groups are collaborating to localize zebrafish genes on an integrated genetic linkage map. This map will accelerate the discovery of gene functions by increasing the number of mapped candidate genes for mutations. Furthermore, genes are uniquely suited for comparative genomics, and we plan to assemble a map of chromosomal segments conserved between zebrafish and human. Previous gene maps were constructed with haploid mapping panels, which simplify linkage analysis but allow only 1000-2000 markers to be scored in each individual (because of the limited genomic DNA available in haploid embryos). To overcome this limitation while retaining the benefit of haploid panels, we are employing a homozygous diploid mapping panel produced by heat shock treatment of the embryonic offspring from two C32/SJD F1 females. Genomic DNA for 10,000-100,000 markers is available from each of the 47 individuals that comprise the panel. By scoring SSCPs linked to genes and ESTs, we plan to create a dense, gene-based map with a resolution of 2 cM (about 1.2 Mb average physical distance). This resolution will allow investigators to identify genes near mutations of interest; any candidate genes in the interval can then be tested in mutant mapping crosses of higher resolution.

Panel Producer: Talbot, William S. Most Recent Update: Mar 2, 2005
Panel type: Meiotic Number of meioses: 42
Current source of genetic material for mapping: Talbot, William S.

HS panel: Statistics

Markers:

    EST (total of 1818)
    CDNA (total of 797)
    SNP (total of 761)
    SSLP (total of 656)
    STS (total of 16)
    GENE (total of 668)
Total of 3955 markers on panel

Click "View Map" in table at right to see a graphical map of an individual chromosome.

OR

Click to see a list of all mapping panels.
ChromosomeMarkers on chromosome
1 SSLP(30) ; CDNA(37) ; SNP(31) ; EST(69) ; GENE(29) Scoring Data
2 EST(95) ; GENE(25) ; SSLP(30) ; CDNA(41) ; SNP(34) Scoring Data
3 CDNA(49) ; GENE(39) ; EST(80) ; SSLP(38) ; STS(1) ; SNP(32) Scoring Data
4 CDNA(19) ; EST(70) ; SSLP(28) ; GENE(6) ; SNP(26) ; STS(1) Scoring Data
5 STS(3) ; GENE(39) ; CDNA(52) ; SSLP(34) ; SNP(59) ; EST(118) Scoring Data
6 EST(73) ; SSLP(30) ; CDNA(43) ; SNP(43) ; GENE(28) Scoring Data
7 SSLP(37) ; EST(102) ; SNP(32) ; CDNA(46) ; GENE(35) Scoring Data
8 EST(71) ; STS(1) ; GENE(26) ; SNP(43) ; SSLP(24) ; CDNA(27) Scoring Data
9 SNP(36) ; EST(69) ; SSLP(21) ; STS(2) ; GENE(32) ; CDNA(33) Scoring Data
10 STS(1) ; CDNA(37) ; SNP(17) ; SSLP(20) ; EST(61) ; GENE(24) Scoring Data
11 CDNA(24) ; GENE(26) ; EST(79) ; SSLP(20) ; SNP(50) Scoring Data
12 SSLP(30) ; CDNA(36) ; EST(56) ; GENE(34) ; SNP(31) Scoring Data
13 CDNA(32) ; SNP(17) ; EST(77) ; GENE(28) ; SSLP(25) Scoring Data
14 EST(58) ; SSLP(29) ; SNP(13) ; CDNA(36) ; GENE(33) Scoring Data
15 SSLP(33) ; EST(72) ; GENE(29) ; SNP(21) ; CDNA(24) Scoring Data
16 SSLP(20) ; SNP(33) ; STS(1) ; EST(75) ; CDNA(24) ; GENE(24) Scoring Data
17 SSLP(16) ; STS(1) ; EST(56) ; SNP(30) ; GENE(24) ; CDNA(20) Scoring Data
18 STS(2) ; CDNA(21) ; EST(63) ; GENE(17) ; SSLP(26) ; SNP(35) Scoring Data
19 GENE(28) ; EST(72) ; CDNA(31) ; SNP(31) ; SSLP(20) Scoring Data
20 CDNA(31) ; SNP(17) ; EST(72) ; GENE(28) ; SSLP(20) Scoring Data
21 EST(77) ; SSLP(33) ; SNP(40) ; CDNA(23) ; GENE(37) Scoring Data
22 SNP(24) ; STS(1) ; CDNA(29) ; SSLP(16) ; EST(67) ; GENE(18) Scoring Data
23 SNP(32) ; STS(1) ; SSLP(28) ; CDNA(36) ; GENE(28) ; EST(86) Scoring Data
24 EST(55) ; SSLP(21) ; GENE(15) ; SNP(13) ; CDNA(23) Scoring Data
25 SSLP(27) ; SNP(21) ; GENE(16) ; EST(45) ; STS(1) ; CDNA(23) Scoring Data
CITATIONS   (3)