PUBLICATION

Molecular analysis of Dec1 and Dec2 in the peripheral circadian clock of zebrafish photosensitive cells

Authors
Abe, T., Ishikawa, T., Masuda, T., Mizusawa, K., Tsukamoto, T., Mitani, H., Yanagisawa, T., Todo, T., and Iigo, M.
ID
ZDB-PUB-061116-9
Date
2006
Source
Biochemical and Biophysical Research Communications   351(4): 1072-1077 (Journal)
Registered Authors
Keywords
Zebrafish, Dec1, Dec2, Clock genes, Circadian rhythm, Peripheral circadian clock, Light
MeSH Terms
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/genetics
  • Basic Helix-Loop-Helix Transcription Factors/physiology*
  • CLOCK Proteins
  • Circadian Rhythm/genetics*
  • Circadian Rhythm/radiation effects
  • Cloning, Molecular
  • Gene Expression Regulation*
  • Light
  • Phylogeny
  • Suppression, Genetic
  • Trans-Activators/genetics
  • Transcription, Genetic/radiation effects
  • Zebrafish/genetics
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
PubMed
17097613 Full text @ Biochem. Biophys. Res. Commun.
Abstract
To elucidate the roles of DEC1 and DEC2, basic helix-loop-helix transcription factors, in the circadian clock of photosensitive zebrafish peripheral cells, zebrafish Dec1 and Dec2 (zDec1 and zDec2) were cloned and their functions and expression patterns were examined in BRF41, a zebrafish cell line. zDEC1 and zDEC2 have high sequence similarity to mammalian counterparts and the molecular phylogenetic analysis of the zDEC1 and zDEC2 sequences reflected the predicted pattern of species classification. zDEC1 and zDEC2 inhibited zCLOCK1:zBMAL3 mediated transcription as CRY1a. zDec1 and zDec2 mRNA showed robust circadian oscillation in BRF41 cells. However, zDec1 and zDec2 mRNA was not strongly induced by exposure to light. These results indicate that zDec1 and zDec2 are involved in the circadian clock mechanism in photosensitive zebrafish peripheral cells by suppressing CLOCK/BMAL-induced gene expression and that the feedback loops of zDEC1 and zDEC2 may be interlocked with the PER/CRY core circadian feedback loops.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping