PUBLICATION
Evaluating the molecular function of zebrafish Anoctamin 1a
- Authors
- Dimatteo, A., Stango, N., Rich, A., Sweet, T.
- ID
- ZDB-PUB-250821-1
- Date
- 2025
- Source
- microPublication. Biology 2025: (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 40838117 Full text @ MicroPubl Biol
Citation
Dimatteo, A., Stango, N., Rich, A., Sweet, T. (2025) Evaluating the molecular function of zebrafish Anoctamin 1a. microPublication. Biology. 2025:.
Abstract
Calcium-activated chloride channels (CaCCs) regulate key physiological processes like epithelial secretion, sensory transduction, gastrointestinal pacemaking, and muscle contraction. Zebrafish ( Danio rerio ) are genetically similar to humans, easily manipulated, and are a valuable model organism. We cloned zebrafish Anoctamin 1, a putative CaCC gene, expressed it in mammalian cells, and confirmed its function via halide flux assays. The channel conducted anions and responded to calcium, verifying its role as a CaCC. These findings confirm the functionality of zebrafish CaCCs and lay the groundwork to use the organism to model human physiology and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping