PUBLICATION

Investigating Psychopharmaceutical Effects on Early Vertebrate Development Using a Zebrafish Model System

Authors
Zimmerman, N., Marta, A., Baker, C., Korade, Z., Mirnics, K., Shibata, A.
ID
ZDB-PUB-250724-17
Date
2025
Source
Journal of developmental biology   13: (Journal)
Registered Authors
Keywords
aripiprazole, cariprazine, cholesterol, psychopharmaceuticals, sterol synthesis, trazodone, zebrafish
MeSH Terms
none
PubMed
40700140 Full text @ J Dev Biol
Abstract
Cholesterol homeostasis is necessary for normal vertebrate development. The disruption of cholesterol homeostasis can cause abnormal body and nervous system development and lead to dysfunctional behavior and increased mortality. Commonly prescribed psychopharmaceuticals can alter cholesterol synthesis and may disrupt early vertebrate development. A high-throughput vertebrate zebrafish model system was used to test the hypothesis that exposure to psychopharmaceutical medications alters cholesterol biosynthesis and disrupts gene transcription, early whole-body and brain development, and nervous system function, resulting in abnormal behavior. Exposure to cariprazine, aripiprazole, trazodone, and AY9944 increased 7-dehydrocholesterol levels compared to vehicle-treated zebrafish. Significant differences in disease-associated gene expression, brain structure, and functional behaviors were observed in psychopharmaceutical and AY9944-treated zebrafish compared to controls. These data reveal that the high-throughput zebrafish model system can discern psychopharmaceutical effects on cholesterol synthesis, gene transcription, and key features of early vertebrate development that influences behavior.
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