PUBLICATION
Sclerotome-derived PDGF signaling functions as a niche cue responsible for primitive erythropoiesis
- Authors
- Mao, A., Li, Z., Ning, G., Zhou, Z., Wei, C., Li, J., He, X., Wang, Q.
- ID
- ZDB-PUB-231118-1
- Date
- 2023
- Source
- Development (Cambridge, England) 150(22): (Journal)
- Registered Authors
- Wang, Qiang
- Keywords
- Cell differentiation, ERK1/2, PDGF, Primitive erythropoiesis, Sclerotome
- MeSH Terms
-
- Animals
- Platelet-Derived Growth Factor*
- Cell Differentiation/genetics
- Zebrafish
- Erythropoiesis*/genetics
- Embryonic Development
- Cues
- PubMed
- 37882745 Full text @ Development
Citation
Mao, A., Li, Z., Ning, G., Zhou, Z., Wei, C., Li, J., He, X., Wang, Q. (2023) Sclerotome-derived PDGF signaling functions as a niche cue responsible for primitive erythropoiesis. Development (Cambridge, England). 150(22):.
Abstract
Primitive erythropoiesis serves a vital role in embryonic development, generating primitive red blood cells responsible for transportation of oxygen throughout the body. Although diverse niche factors are known to function in definitive hematopoiesis, the microenvironment contributing to primitive hematopoiesis remains largely elusive. Here, we report that platelet-derived growth factor (PDGF) signaling is required for erythroid progenitor differentiation in zebrafish. Ablating pdgfαa (also known as pdgfaa) and pdgfαb (also known as pdgfab) or blocking PDGF signaling with an inhibitor impairs erythroid progenitor differentiation, thus resulting in a significant decrease in the number of erythrocytes. We reveal that pdgfαb is expressed in sclerotomal cells, and that its receptor genes, pdgfra and pdgfrb, are expressed in the adjacent erythroid progenitor cells. Sclerotome-specific overexpression of pdgfαb effectively restores primitive erythropoiesis in pdgfαa-/-;pdgfαb-/- mutant embryos. In addition, we have defined ERK1/2 signaling as a downstream pathway of PDGF signaling during embryonic erythropoiesis. Taken together, our findings indicate that PDGF signaling derived from sclerotome functions as a niche cue for primitive erythropoiesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping